Coding
OMT-RS

Part:BBa_K1976020:Design

Designed by: Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Schäfer, Inka Schröter, Viktoria Schuster   Group: iGEM16_TU_Darmstadt   (2016-10-13)


O-Methyl-L-tyrosine tRNA synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesis feasible by IDT, no amber stop codon for termination


Source

Methanocaldococcus jannaschii (optimized via directed evolution)

References

[1] L. Wang, J. Xie and P. G. Schultz, Expanding the genetic code, Annu Rev Biophys, vol. 35, pp. 225-249, 2006
[2] L. Wang, A. Brock, B. Herberich and P. G. Schultz, Expanding the genetic code of Escherichia coli, Science, vol. 292, pp.498-500, 2001
[3] C. C. Liu and P. G. Schultz, Adding new chemistries to the genetic code, Annu Rev Biochem, vol. 79, pp.413-444, 2010
[4] Y. Zhang, L. Wang, P. G. Schultz and I. A. Wilson, Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine, Protein Sci, vol. 14, pp.1340-1349, 2005